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Proteins were then precipitated by the addition of the total sample volume to 4 volume liquid chromatographymass spectrometry (LCMS)-grade acetonitrile, as per the SP4 protocol 63 , followed by gentle shaking for 5 min and centrifugation at 3,220 g for 10 min

It is also a co-factor for several metabolic enzymes is involved in intracellular transport, functions as an antioxidant and radioprotectant and facilitates protein folding and degradation

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Liposomal glutathione, however, bypasses much of this degradation

10 The first step in the biosynthesis of GSH is rate-limiting and is catalysed by glutamate-cysteine ligase (GCL)