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The coding sequence of full-length potential interactors was cloned into the prey vector pGADT7 and co-transformed with pGBKT7-SsCm1 SP into Y2HGold yeast cells

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If any of the following happen, tell your nurse immediately: Chest tightness, shortness of breath, or wheezing Hives or swelling of the lips or tongue Severe back or abdominal pain Intense pain, swelling, skin coolness, blanching, or burning at the IV site (signs of infiltration , where fluid leaks into surrounding tissue) A quality provider keeps emergency medications, oxygen, and clear EMS escalation protocols available for exactly this reason

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