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Importantly, NC-6300 normalizes the TME by increasing blood vessel maturity and reducing fibrosis, leading to increased intratumor density and proliferation of T cells when combined with checkpoint inhibition

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After centrifugation, 10 l of the supernatant were filtered through a Microcon YM-10 centrifugal filter device (Amicon), and analyzed by reversed-phase high-performance liquid chromatography on a C18 column (Atlantis 5 m, 4,6200 mm, Waters) as previously described [18]