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doi:10.1016/S0304-3940(02)00982-5 8
Add 20 L of diluted standard or sample to 0.5 mL centrifuge tube, add 1 L of Reagent II, incubate at 37 for 30 minutes after mixing
"Review resumes beforehand," Marita recommends
Most devices require holding the button down until the dose is completely delivered, which may be indicated by a click, counter, or window marker
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All subsequent values are presented as least square (LS) means with additional standard errors
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